A tobacco matrix attachment region reduces the loss of transgene expression in the progeny of transgenic tobacco plants
نویسندگان
چکیده
منابع مشابه
Effect of nuclear matrix attachment regions on transgene expression in tobacco plants.
Matrix attachment regions (MARs) are thought to participate in the organization and segregation of independent chromosomal loop domains. Although there are several reports on the action of natural MARs in the context of heterologous genes in transgenic plants, in our study we tested a synthetic MAR (sMAR) with the special property of unpairing when under superhelical strain, for its effect on r...
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Many studies in both plant and animal systems have shown that matrix attachment regions (MARs) can increase expression of transgenes in whole organisms or cells in culture. Because histochemical assays often indicate variegated transgene expression, a question arises: Do MARs increase transgene expression by increasing the percentage of cells expressing the transgene (likelihood), by increasing...
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We have previously shown that yeast scaffold attachment regions (SARs) flanking a chimeric beta-glucuronidase (GUS) reporter gene increased per-copy expression levels by 24-fold in tobacco suspension cell lines stably transformed by microprojectile bombardment. In this study, we examined the effect of a DNA fragment originally identified in a tobacco genomic clone by its activity in an in vitro...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1999
ISSN: 0960-7412
DOI: 10.1046/j.1365-313x.1999.00453.x